Coding

Part:BBa_K3137006:Design

Designed by: Jiamin Chen   Group: iGEM19_JiangnanU_China   (2019-10-07)


abpAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal BglII site found at 561
    Illegal BglII site found at 1621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
    Illegal NgoMIV site found at 3333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequences of abpA and abpB overlap by 4 bp, which may facilitate the coupling of their expression at the translational level.

Source

AbpA and abpB are obtained by PCR from the genome of E.coli BL21.

References

Yasui R , Washizaki A , Furihata Y , et al. AbpA and AbpB provide anti-phage activity in Escherichia coli[J]. Genes & Genetic Systems, 2014, 89(2):51-60.